CPC G01N 33/6896 (2013.01) [G01N 2800/2814 (2013.01); G01N 2800/2835 (2013.01)] | 19 Claims |
1. An in vitro method for detecting the presence of alpha-synuclein (α-syn) aggregate in a biological sample, the method comprising:
(A) providing a biological sample;
(B) providing a pre-incubation mixture, the pre-incubation mixture comprising:
(1) a monomeric α-syn protein;
(2) a buffer composition;
(3) a salt;
(4) a fluorescent protein aggregation indicator; and
(5) one or more Si3N4 beads having a diameter of 1 mm to 5 mm;
(C) combining the biological sample and the pre-incubation mixture to form an incubation mixture;
(D) incubating and agitating the incubation mixture to form an incubated mixture;
(E) illuminating the incubated mixture with a wavelength of light that excites the fluorescent protein aggregation indicator; and
(F) determining a level of fluorescence during incubation,
wherein an increase in the level of fluorescence during incubation indicates the presence of α-syn aggregate in the biological sample.
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