	US 11,959,910 B2
	Compositions and methods for disease diagnosis using single cell analysis
Nicolas Arab, Austin, TX (US); and Ross Johnson, Austin, TX (US)
Assigned to Pattern Bioscience, Inc., Austin, TX (US)
Filed by PATTERN BIOSCIENCE, INC., Austin, TX (US)
Filed on Jul. 12, 2021, as Appl. No. 17/372,957.
Application 17/372,957 is a continuation of application No. 16/656,962, filed on Oct. 18, 2019, granted, now 11,061,018.
Application 16/656,962 is a continuation of application No. 15/833,629, filed on Dec. 6, 2017, granted, now 10,488,404, issued on Nov. 26, 2019.
Application 15/833,629 is a continuation of application No. 15/466,377, filed on Mar. 22, 2017, granted, now 9,851,345, issued on Dec. 26, 2017.
Claims priority of provisional application 62/407,311, filed on Oct. 12, 2016.
Prior Publication US 2022/0137029 A1, May 5, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/04 (2006.01); C12Q 1/18 (2006.01); G01N 15/14 (2006.01); G01N 21/64 (2006.01); G01N 33/50 (2006.01); G01N 33/58 (2006.01); G01N 15/10 (2006.01); G01N 15/1404 (2024.01)

CPC G01N 33/5091 (2013.01) [C12Q 1/04 (2013.01); C12Q 1/18 (2013.01); G01N 15/1463 (2013.01); G01N 21/6428 (2013.01); G01N 33/5011 (2013.01); G01N 33/582 (2013.01); G01N 2015/1006 (2013.01); G01N 2015/1406 (2013.01); G01N 2015/1481 (2013.01); G01N 2201/0621 (2013.01); G01N 2800/26 (2013.01)]

10 Claims

1. A method for identifying a cell comprising:

(a) contacting a sample containing cells with at least two different reporters having identical emission spectra, thereby forming a sample mixture;

(b) partitioning the sample mixture into a plurality of volume partitions containing one cell or one cellular aggregate;

(d) monitoring optical characteristics of the volume partitions during the incubation time;

(e) constructing waveforms for the volume partitions based on the optical characteristics over time; and

(f) identifying the cell in the volume partition based on the waveform constructed for the volume partition.