US 11,959,838 B2
Representative diagnostics
Nelson Alexander, Marana, AZ (US); Aoune Barhoumi, Tucson, AZ (US); Melinda Day, Oro Valley, AZ (US); Lisa Gallegos, Tucson, AZ (US); Katherine Leith, Tucson, AZ (US); Samantha Rajkovich, Marana, AZ (US); Esteban Roberts, Tucson, AZ (US); Stacey Stanislaw, Tucson, AZ (US); and Eric Walk, Tucson, AZ (US)
Assigned to Ventana Medical Systems, Inc., Tucson, AZ (US)
Filed by Ventana Medical Systems, Inc., Tucson, AZ (US)
Filed on Apr. 27, 2018, as Appl. No. 15/965,786.
Application 15/965,786 is a continuation of application No. PCT/US2016/060861, filed on Nov. 7, 2016.
Claims priority of provisional application 62/418,146, filed on Nov. 4, 2016.
Claims priority of provisional application 62/354,622, filed on Jun. 24, 2016.
Claims priority of provisional application 62/279,405, filed on Jan. 15, 2016.
Claims priority of provisional application 62/252,153, filed on Nov. 6, 2015.
Prior Publication US 2020/0049599 A1, Feb. 13, 2020
Int. Cl. G01N 1/28 (2006.01); A01N 1/02 (2006.01); G01N 1/30 (2006.01); G01N 1/36 (2006.01); G01N 33/483 (2006.01)
CPC G01N 1/286 (2013.01) [A01N 1/0226 (2013.01); A01N 1/0278 (2013.01); G01N 1/30 (2013.01); G01N 1/36 (2013.01); G01N 33/4833 (2013.01); G01N 2001/2866 (2013.01); G01N 2001/368 (2013.01)] 43 Claims
 
1. A method for preparing a representative sample for analysis, comprising:
a) obtaining a surgical resection tissue sample from a tumor of a subject, wherein the obtained surgical resection tissue sample is at least 1 cm in diameter at its widest point, and wherein the obtained surgical resection tissue sample is fixed; and wherein the obtained surgical resection tissue sample is not derived from a formalin-fixed paraffin-embedded sample; and
b) mechanically blending the obtained surgical resection tissue sample to provide the representative sample, wherein any heterogeneity of cells within the obtained surgical resection tissue sample is substantially homogeneously distributed within the representative sample, and wherein any aliquot removed from the representative sample comprises one or more populations of subclones at a proportion at which they existed within the obtained surgical resection tissue sample.