	US 11,959,120 B2
	Method of making lipids with improved cold flow properties
Zhiyong Sun, Dartmouth (CA); Alan Sothern, Dartmouth (CA); Kevin Berryman, Dartmouth (CA); Mercia Valentine, Dartmouth (CA); Michael Milway, Dartmouth (CA); Laura Purdue, Dartmouth (CA); and Roberto E. Armenta, Dartmouth (CA)
Assigned to MARA Renewables Corporation, Dartmouth (CA)
Filed by MARA RENEWABLES CORPORATION, Dartmouth (CA)
Filed on Oct. 28, 2020, as Appl. No. 17/083,045.
Application 17/083,045 is a continuation of application No. 15/178,890, filed on Jun. 10, 2016, granted, now 10,851,395.
Prior Publication US 2021/0040519 A1, Feb. 11, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 7/6427 (2022.01); C12P 7/6463 (2022.01)

CPC C12P 7/6427 (2013.01) [C12P 7/6463 (2013.01)]

23 Claims

1. A method of producing a microbial oil comprising fatty acids, wherein the method comprises:

culturing oil-producing Thraustochytrid microorganisms in a fermentation medium in the presence of one or more antifoaming agents continuously added to the fermentation medium under a controlled carbon consumption rate of between 1.5 and 4.5 g/L per hour, wherein the culturing produces an oil comprising:

(a) saturated fatty acids, wherein 20% to 35% of the fatty acids are saturated fatty acids;

(b) omega-7 fatty acids, wherein 10-30% of the fatty acids are omega-7 fatty acids comprising C16:1 (n-7) palmitoleic acid and C18:1 (n-7) cis-vaccenic acid, and