US 11,959,108 B2
Engineered protease variants
Chinping Chng, Menlo Park, CA (US); Nikki Dellas, San Carlos, CA (US); Ravi David Garcia, Los Gatos, CA (US); Moulay Hicham Alaoui Ismaili, San Mateo, CA (US); Kristen Jean Vallieu, Union City, CA (US); and Kerryn McCluskie, Pacifica, CA (US)
Assigned to Codexis, Inc., Redwood City, CA (US); and Societe des Produits Nestle S.A., Vevey (CH)
Filed by Codexis, Inc., Redwood City, CA (US); and Societe des Produits Nestle S.A., Vevey (CH)
Filed on Aug. 27, 2021, as Appl. No. 17/460,141.
Claims priority of provisional application 63/071,625, filed on Aug. 28, 2020.
Prior Publication US 2022/0090039 A1, Mar. 24, 2022
Int. Cl. C12N 9/48 (2006.01); A61K 38/48 (2006.01); A61P 1/18 (2006.01)
CPC C12N 9/48 (2013.01) [A61K 38/48 (2013.01); A61P 1/18 (2018.01)] 39 Claims
 
1. A recombinant protease comprising an amino acid sequence comprising at least 85% sequence identity to SEQ ID NO: 2, wherein the amino acid sequence of the recombinant protease comprises at least a substitution at amino acid position 236, 258, 261, 339, 439, 446, or 450, wherein the amino acid positions are numbered with reference to SEQ ID NO: 2.