CPC C12N 15/8221 (2013.01) [A01H 1/00 (2013.01); A01H 5/00 (2013.01); C12N 15/8234 (2013.01); C12N 15/8274 (2013.01)] | 9 Claims |
1. A recombinant chimeric nucleic acid molecule comprising a nucleic acid sequence encoding a dual transit peptide operably linked to a heterologous nucleic acid sequence encoding a heterologous polypeptide of interest,
wherein said dual transit peptide is from the genus Amaranthus and translocates the polypeptide of interest to a chloroplast or a mitochondrion,
wherein said dual transit peptide comprises an amino acid sequence having 100% sequence identity to SEQ ID NO: 1,
wherein said heterologous polypeptide of interest is a polypeptide which, when overexpressed in a plant, confers herbicide tolerance to said plant,
wherein said heterologous polypeptide of interest is from a prokaryote,
wherein said polypeptide has protoporphyrinogen oxidase (PPO) activity and confers tolerance to PPO-inhibiting herbicides, and
wherein said polypeptide having PPO activity comprises an amino acid sequence having 100% sequence identity to SEQ ID NO: 320.
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