US 11,959,086 B2
Dual transit peptides for targeting polypeptides
Raphael Aponte, Limburgerhof (DE); Stefan Tresch, Ludwigshafen (DE); Tobias Seiser, Limburgerhof (DE); and Jill M. Paulik, Research Triangle Park, NC (US)
Assigned to BASF AGRO B.V., Arnhem (NL)
Appl. No. 16/302,202
Filed by BASF AGRO B.V., Arnhem (NL)
PCT Filed May 19, 2017, PCT No. PCT/EP2017/062182
§ 371(c)(1), (2) Date Nov. 16, 2018,
PCT Pub. No. WO2017/198859, PCT Pub. Date Nov. 23, 2017.
Claims priority of application No. 16170705 (EP), filed on May 20, 2016.
Prior Publication US 2019/0169626 A1, Jun. 6, 2019
Int. Cl. A01H 1/00 (2006.01); A01H 5/00 (2018.01); C12N 15/82 (2006.01)
CPC C12N 15/8221 (2013.01) [A01H 1/00 (2013.01); A01H 5/00 (2013.01); C12N 15/8234 (2013.01); C12N 15/8274 (2013.01)] 9 Claims
 
1. A recombinant chimeric nucleic acid molecule comprising a nucleic acid sequence encoding a dual transit peptide operably linked to a heterologous nucleic acid sequence encoding a heterologous polypeptide of interest,
wherein said dual transit peptide is from the genus Amaranthus and translocates the polypeptide of interest to a chloroplast or a mitochondrion,
wherein said dual transit peptide comprises an amino acid sequence having 100% sequence identity to SEQ ID NO: 1,
wherein said heterologous polypeptide of interest is a polypeptide which, when overexpressed in a plant, confers herbicide tolerance to said plant,
wherein said heterologous polypeptide of interest is from a prokaryote,
wherein said polypeptide has protoporphyrinogen oxidase (PPO) activity and confers tolerance to PPO-inhibiting herbicides, and
wherein said polypeptide having PPO activity comprises an amino acid sequence having 100% sequence identity to SEQ ID NO: 320.