CPC C12N 15/67 (2013.01) [C07K 14/70503 (2013.01); C07K 14/7051 (2013.01); C07K 2319/02 (2013.01); C07K 2319/03 (2013.01)] | 21 Claims |
1. A nucleic acid construct comprising:
a first nucleotide sequence of interest (NOI1), a translational readthrough motif (TRM), and a second nucleotide sequence of interest (NOI2), and
a nucleotide sequence encoding a cleavage site (CL), so that NOI1 and NOI2 are expressed as separate proteins,
wherein the (TRM) comprises the sequence STOP-CUAG or STOP-CAAUUA, in which “STOP” is a stop codon.
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10. A nucleic acid construct comprising:
a first nucleotide sequence of interest (NOI1), a translational readthrough motif (TRM), and a second nucleotide sequence of interest (NOI2), and
a nucleotide sequence encoding a cleavage site (CL), so that NOI1 and NOI2 are expressed as separate proteins,
wherein NOI2 encodes a cytokine, chemokine or toxin.
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15. A nucleic acid construct comprising: a first nucleotide sequence of interest (NOI1), a translational readthrough motif (TRM) and a second nucleotide sequence of interest (NOI2),
wherein the nucleic acid construct is capable of producing two products when expressed in a cell:
a) a first product encoded by NOI1 alone, and
b) a second product, encoded by NOI1 and NOI2, which is produced when translational readthrough occurs, and
wherein the first and/or second product is a chimeric antigen receptor (CAR).
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