US 11,959,062 B2
Distributed systems for the efficient production and use of microbe-based compositions
Sean Farmer, Ft. Lauderdale, FL (US); Paul S. Zorner, Encinitas, CA (US); Ken Alibek, Solon, OH (US); Kent Adams, Oro Valley, AZ (US); and Tyler Dixon, Madison, OH (US)
Assigned to LOCUS SOLUTIONS IPCO, LLC, Solon, OH (US)
Filed by LOCUS SOLUTIONS IPCO, LLC, Solon, OH (US)
Filed on Aug. 10, 2023, as Appl. No. 18/447,387.
Application 18/447,387 is a continuation of application No. 16/331,438, granted, now 11,760,969, previously published as PCT/US2017/050711, filed on Sep. 8, 2017.
Claims priority of provisional application 62/385,057, filed on Sep. 8, 2016.
Prior Publication US 2023/0383229 A1, Nov. 30, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 1/14 (2006.01); C12M 1/00 (2006.01); C12M 1/34 (2006.01); C12M 3/00 (2006.01); C12N 1/16 (2006.01); C12N 1/20 (2006.01); C12P 7/64 (2022.01); C12P 19/44 (2006.01)
CPC C12M 41/26 (2013.01) [C12M 23/44 (2013.01); C12M 23/52 (2013.01); C12M 23/58 (2013.01); C12M 41/12 (2013.01); C12N 1/14 (2013.01); C12N 1/16 (2013.01); C12N 1/20 (2013.01); C12P 7/64 (2013.01); C12P 19/44 (2013.01); Y02A 40/10 (2018.01)] 15 Claims
 
1. A method for providing a microbe-based product selected from the group consisting of microbes, a broth in which the microbes were grown, a biosurfactant produced by the microbes, and combinations thereof, wherein said method comprises growing the microbes in a microbe growth facility, harvesting said microbes, broth and/or biosurfactant, creating said microbe-based product from said harvested microbes, broth and/or biosurfactant, and transporting the microbe-based product to a location for use;
wherein the biosurfactant is selected from the group consisting rhamnolipids, sophorolipids (SLP) and mannosylerythritol lipids (MEL);
wherein said microbes are selected from the group consisting of Pseudomonas aeruginosa, Starmerella bombicola, Pichia anomala, Wickerhamomyces anomalus, Trichoderma harzianum, Bacillus amyloliquefaciens, Bacillus subtilis, and Pseudozyma aphidis;
wherein said microbe growth facility comprises a plurality of independent modular growth vessels in which the same, or different, microbes grow, under the same, or different, growth conditions that can be varied between the independent growth vessels;
wherein at least one of the growth conditions that can be varied between the independent modular growth vessels is selected from the group consisting of growth medium, oxygenation, pH, agitation, and temperature; and
wherein said transport of the microbe-based product is in a container having a volume of 1 gallon to 1,000 gallons, or via a conduit.