US 11,959,056 B2
Cell culture system
Robert G. Pleydon, Worcester, MA (US); Natasha Anna Boghosian, Boston, MA (US); Jacob Andrews, Washington, DC (US); and Phillippe Jean Broussard, Finksburg, MD (US)
Assigned to Saint-Gobain Performance Plastics Corporation, Solon, OH (US)
Filed by Saint-Gobain Performance Plastics Corporation, Solon, OH (US)
Filed on Jun. 30, 2020, as Appl. No. 16/917,331.
Claims priority of provisional application 62/869,021, filed on Jun. 30, 2019.
Prior Publication US 2020/0407670 A1, Dec. 31, 2020
Int. Cl. C12M 1/00 (2006.01); C12N 1/04 (2006.01); C12N 5/0786 (2010.01)
CPC C12M 23/14 (2013.01) [C12M 29/00 (2013.01); C12N 1/04 (2013.01); C12N 5/0645 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A bag comprising:
one or more polymer films, having edges bonded together to form edges around an undivided interior compartment of the bag;
a first port formed in an exterior surface of the bag;
a first liquid-permeable tube extending into and in fluid communication with the interior compartment of the bag, the first liquid-permeable tube being operatively coupled to the first port;
a second port formed in an exterior surface of the bag;
a second liquid-permeable tube extending into and in fluid communication with the interior compartment of the bag, the second liquid-permeable tube being operatively coupled to the second port; and
optionally, a third port formed in an exterior surface of the bag, the third port being in fluid communication with the interior compartment of the bag;
wherein the first liquid-permeable tube comprises:
a first inner support structure defining a central lumen of the first liquid-permeable tube, wherein the first inner support structure comprises a first porous tube having an average pore size in the range of 250 to 5000 μm; and
a first outer filter layer surrounding the first inner support structure, wherein the first outer filter layer has an average pore size of less than 50 μm.