CPC C07H 19/14 (2013.01) [C07H 19/10 (2013.01); C07H 19/20 (2013.01); C12Q 1/6869 (2013.01); C12Q 1/6876 (2013.01)] | 21 Claims |
1. A kit comprising a labeled nucleotide, wherein the labeled nucleotide comprises:
(i) a cleavable moiety covalently bound to the 3′ oxygen of said labeled nucleotide, wherein the cleavable moiety has the formula -L1P-S—S—R6P(I); and
(ii) a nucleobase linked via a covalent linker to a first detectable moiety, wherein said covalent linker comprises
wherein,
R102 is unsubstituted C1-C4 alkyl;
L1P is a substituted methylene, wherein L1P is substituted with a substituted or unsubstituted alkyl, or a substituted or unsubstituted heteroalkyl; and
R6P is unsubstituted methyl or unsubstituted ethyl.
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