US 11,957,690 B2
Chromogenic beta-lactamase substrate
Anita Shukla, Providence, RI (US); Dahlia Alkekhia, Providence, RI (US); Shashank Shukla, Billerica, MA (US); and Hannah Safford, Philadelphia, PA (US)
Assigned to BROWN UNIVERSITY, Providence, RI (US)
Appl. No. 17/260,894
Filed by BROWN UNIVERSITY, Providence, RI (US)
PCT Filed Jul. 15, 2019, PCT No. PCT/US2019/041884
§ 371(c)(1), (2) Date Jan. 15, 2021,
PCT Pub. No. WO2020/018462, PCT Pub. Date Jan. 23, 2020.
Claims priority of provisional application 62/698,695, filed on Jul. 16, 2018.
Prior Publication US 2021/0252010 A1, Aug. 19, 2021
Int. Cl. A61K 31/545 (2006.01); A61K 47/54 (2017.01); A61K 47/60 (2017.01); A61K 47/61 (2017.01); A61K 47/69 (2017.01); A61P 31/04 (2006.01); A61K 31/546 (2006.01)
CPC A61K 31/545 (2013.01) [A61K 47/545 (2017.08); A61K 47/60 (2017.08); A61K 47/61 (2017.08); A61K 47/6903 (2017.08); A61P 31/04 (2018.01); A61K 31/546 (2013.01)] 3 Claims
 
1. A diagnostic agent that changes color in response to presence of beta lactamases, wherein the diagnostic agent consists of the following formula:

OG Complex Work Unit Chemistry
wherein the maleimide moiety in the structure above facilitates conjugation to a hydrogel forming polymer; further wherein the polymer is not an oligosaccharide; and wherein the diagnostic color change response is from clear to yellow when the B-lactam ring is hydrolyzed, expelling the 4-nitrobenzenethiol leaving group.