US 11,957,102 B2
Plant with altered content of steroidal alkaloids
Asaph Aharoni, Tel Aviv (IL); Prashant Sonawane, Rehovot (IL); and Maxim Itkin, Rehovot (IL)
Assigned to YEDA RESEARCH AND DEVELOPMENT CO. LTD., Rehovot (IL)
Filed by YEDA RESEARCH AND DEVELOPMENT CO. LTD., Rehovot (IL)
Filed on Jul. 7, 2022, as Appl. No. 17/859,053.
Application 17/859,053 is a division of application No. 17/008,875, filed on Sep. 1, 2020, granted, now 11,412,700.
Application 17/008,875 is a division of application No. 16/123,248, filed on Sep. 6, 2018, granted, now 10,806,119.
Application 16/123,248 is a continuation in part of application No. 14/895,059, granted, now 10,100,322, previously published as PCT/IL2014/050497, filed on Jun. 2, 2014.
Claims priority of provisional application 61/831,164, filed on Jun. 5, 2013.
Prior Publication US 2022/0408679 A1, Dec. 29, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. A01H 6/82 (2018.01); C12N 9/10 (2006.01); C12N 15/82 (2006.01)
CPC A01H 6/82 (2018.05) [C12N 9/1059 (2013.01); C12N 15/8218 (2013.01); C12N 15/8243 (2013.01); C12N 15/8247 (2013.01)] 32 Claims
 
1. A genetically modified plant comprising at least one cell having altered expression of a gene encoding a GAME15 cellulose synthase like protein compared to its expression in a corresponding unmodified plant, wherein the genetically modified plant has an altered content of at least one steroidal alkaloid or a glycosylated derivative thereof, or of at least one steroidal saponin or a glycosylated derivative thereof, compared to the corresponding unmodified plant, wherein
(a) the amino acid sequence of the cellulose synthase like protein of the corresponding unmodified plant comprises amino acid sequence set forth in any one of SEQ ID NOS: 33, 35, 37, 39, 42, or 43; or
(b) the polynucleotide encoding the cellulose synthase like protein of the corresponding unmodified plant comprises the nucleic acid sequence set forth in any one of SEQ ID NOS: 32, 34, 36, 38, 40, or 42.