US 12,277,701 B2
Assay reading method
Neeraj Adsul, York (GB); and Marcin Tokarzewski, York (GB)
Assigned to FORSITE DIAGNOSTICS LIMITED, Avon (GB)
Appl. No. 17/628,490
Filed by FORSITE DIAGNOSTICS LIMITED, Avon (GB)
PCT Filed Apr. 22, 2020, PCT No. PCT/EP2020/061253
§ 371(c)(1), (2) Date Jan. 19, 2022,
PCT Pub. No. WO2021/013392, PCT Pub. Date Jan. 28, 2021.
Claims priority of application No. 1910409 (GB), filed on Jul. 19, 2019.
Prior Publication US 2022/0254133 A1, Aug. 11, 2022
Int. Cl. G06T 7/00 (2017.01); G01N 33/543 (2006.01); G06V 10/22 (2022.01); G06V 10/44 (2022.01); G06V 10/82 (2022.01)
CPC G06T 7/0012 (2013.01) [G01N 33/54388 (2021.08); G06V 10/225 (2022.01); G06V 10/454 (2022.01); G06V 10/82 (2022.01)] 17 Claims
OG exemplary drawing
 
1. A computer implemented method for reading a test region of an assay, the method comprising:
(i) receiving digital image data of a first assay, the digital image data comprising at least one region of pixels corresponding to a first test region of a first assay and at least one region of pixels corresponding to a housing of the first assay;
(ii) inputting the digital image data into a trained convolutional neural network configured to output, for the input digital image data:
a first region of pixels of the input digital image data corresponding to the first test region of the assay, and a first probability that the first region of pixels corresponds to the first test region; and
a second region of pixels of the input digital image data corresponding to the housing of the first assay, and a second probability that the second region of pixels corresponds to the housing,
(iii) if the first probability is at or above a first predetermined threshold, reading the first test region by estimating an intensity value of at least a portion of the first test region in the first region of pixels,
wherein estimating the intensity value comprises performing thresholding on the pixels of the first region of pixels and the second region of pixels, wherein said thresholding comprising replacing any pixel value in the input digital image data below a predetermined threshold value to a first new value, and any pixel value above the threshold to a second new value;
or otherwise discarding the first region of pixels; and
(iv) when the second probability is at or above a second threshold value, determining that the first test region is associated with the first assay by determining that the first region of pixels is within the second region of pixels.