US 12,276,669 B2
Systems and methods for diagnosing and treating chronic organ rejection
Thalachallour Mohanakumar, Phoenix, AZ (US)
Assigned to Dignity Health, San Francisco, CA (US)
Filed by Dignity Health, San Francisco, CA (US)
Filed on Apr. 23, 2020, as Appl. No. 16/856,418.
Claims priority of provisional application 62/841,076, filed on Apr. 30, 2019.
Prior Publication US 2020/0348317 A1, Nov. 5, 2020
Int. Cl. A61K 9/12 (2006.01); G01N 33/68 (2006.01)
CPC G01N 33/6893 (2013.01) [A61K 9/12 (2013.01); G01N 2333/78 (2013.01); G01N 2800/245 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A method of treating chronic organ rejection in a subject having previously received an allographic organ transplant, the method comprising:
a) obtaining or having obtained a first plasma sample from the subject at a first time point, wherein the subject does not yet experience any symptom of chronic organ rejection;
b) isolating from the first plasma sample one or more exosomes;
c) measuring expression of K-alpha 1 tubulin (Ka1T) and Collagen-V (Col-V) within the one or more exosomes;
d) repeating steps of a) through c) in a fixed cadence;
e) obtaining or having obtained a last plasma sample from the subject, at a last time point about 6 months from the first time point;
f) treating the subject with at least one therapeutic methodology when
(i) Col-V AUC value is at least about 0.81; or
(ii) Ka1T AUC value is at least about 0.82.