US 12,276,659 B2
Compositions and methods for passive optical barcoding for multiplexed assays
Jeffrey Kim, Berkeley, CA (US); Anh Tuan Nguyen, San Francisco, CA (US); and Brandon Miller, Oakland, CA (US)
Assigned to Slingshot Biosciences, Inc., Emeryville, CA (US)
Filed by Slingshot Biosciences, Inc., Emeryville, CA (US)
Filed on Feb. 3, 2023, as Appl. No. 18/105,582.
Application 18/105,582 is a division of application No. 17/307,127, filed on May 4, 2021, granted, now 11,598,768.
Claims priority of provisional application 63/019,478, filed on May 4, 2020.
Prior Publication US 2023/0176042 A1, Jun. 8, 2023
Int. Cl. G01N 33/543 (2006.01); C08J 3/075 (2006.01); G01N 15/1434 (2024.01); G01N 33/53 (2006.01)
CPC G01N 33/5432 (2013.01) [C08J 3/075 (2013.01); G01N 15/1434 (2013.01); G01N 33/53 (2013.01); G01N 33/54313 (2013.01); C08J 2333/26 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A composition for demultiplexing one or more targets based on an encoded optical property, said composition comprising:
a plurality of populations of hydrogel particles, each population of hydrogel particles having substantially the same average particle diameter,
wherein each population of hydrogel particles comprises a different forward and/or side scatter optical property, so as to be optically distinguishable from other populations of hydrogel particles within the composition;
wherein each population of hydrogel particles comprises a different functionalized ligand configured to form a particle target combination with a target selected from the group consisting of a chemical, cellular, or biochemical target;
wherein each population of the plurality of hydrogel particles is configured to allow for simultaneous demultiplexing of the one or more of the target.