US 12,276,658 B2
Compositions and methods for rapid and reversible biomolecular labeling
Samuel Jon Clarke, Vancouver (CA)
Assigned to StemCell Technologies Canada Inc., Vancouver (CA)
Appl. No. 14/419,665
Filed by STEMCELL TECHNOLOGIES INC., Vancouver (CA)
PCT Filed Aug. 22, 2013, PCT No. PCT/CA2013/000733
§ 371(c)(1), (2) Date Feb. 5, 2015,
PCT Pub. No. WO2014/029012, PCT Pub. Date Feb. 27, 2014.
Claims priority of provisional application 61/781,651, filed on Mar. 14, 2013.
Claims priority of provisional application 61/692,422, filed on Aug. 23, 2012.
Prior Publication US 2015/0204857 A1, Jul. 23, 2015
Int. Cl. G01N 33/533 (2006.01); B01D 15/26 (2006.01); B82Y 5/00 (2011.01); G01N 33/543 (2006.01); G01N 33/569 (2006.01); G01N 33/58 (2006.01)
CPC G01N 33/533 (2013.01) [B01D 15/265 (2013.01); G01N 33/54306 (2013.01); G01N 33/56966 (2013.01); G01N 33/58 (2013.01); B82Y 5/00 (2013.01); G01N 2333/7155 (2013.01)] 20 Claims
 
1. A method of separating target cells from a label in a sample comprising:
1) Binding the target cells to the label through a linking system comprising (i) an antibody that binds to the target cells, (ii) an antibody that binds to a first polymer, and (iii) a label either conjugated with the first polymer or with the antibody that binds to the first polymer, the first polymer having a first molecular weight;
2) Separating the target cells bound to the label from the sample to enrich a first population of the target cells;
3) contacting the enriched cells of step 2) with a second polymer, the second polymer having a second molecular weight and specifically reversing the interaction between the antibody that binds to the first polymer and the first polymer, wherein a ratio of the first molecular weight and the second molecular weight is 10:1 or less; and
4) separating label from the target cells to obtain a second population of the target cells with increased cell purity compared to the first population of the target cells, wherein a percentage of the target cells in the second population is higher than a percentage of the target cells in the first population.