US 12,276,645 B2
Methods for quantification of carbohydrates
Rajendar Burki, Telangana (IN); Ramesh Venkat Matur, Telangana (IN); Narender Dev Mantena, Telangana (IN); and Mahima Datla, Telangana (IN)
Assigned to Biological E Limited, (IN)
Appl. No. 17/764,351
Filed by Biological E Limited, Telangana (IN)
PCT Filed Sep. 30, 2020, PCT No. PCT/IN2020/050841
§ 371(c)(1), (2) Date Mar. 28, 2022,
PCT Pub. No. WO2021/064748, PCT Pub. Date Apr. 8, 2021.
Claims priority of application No. 201941039796 (IN), filed on Oct. 1, 2019.
Prior Publication US 2022/0390423 A1, Dec. 8, 2022
Int. Cl. G01N 31/22 (2006.01); G01N 21/31 (2006.01); G01N 21/78 (2006.01)
CPC G01N 31/22 (2013.01) [G01N 21/31 (2013.01); G01N 21/78 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A method for quantification of carbohydrates in an aqueous sample, said method comprising the steps of:
a. admixing sulphuric acid and 2-Phenoxyethanol with an aqueous sample containing carbohydrates to obtain a reaction mixture;
b. incubating the reaction mixture to form a coloured complex; and
c. measuring the absorbance of the coloured complex to quantify an amount of carbohydrates present in the aqueous sample.