US 12,276,601 B2
Method for measuring the absorbance of a substance in a solution and a measuring device therefor
Mikael Anders Hornqvist, Uppsala (SE)
Assigned to Cytiva Sweden AB, Uppsala (SE)
Filed by Cytiva Sweden AB, Uppsala (SE)
Filed on Oct. 4, 2023, as Appl. No. 18/481,117.
Application 18/481,117 is a continuation of application No. 16/316,276, granted, now 11,815,451, previously published as PCT/EP2017/065912, filed on Jun. 27, 2017.
Claims priority of application No. 1612010 (GB), filed on Jul. 11, 2016.
Prior Publication US 2024/0027337 A1, Jan. 25, 2024
Int. Cl. G01N 21/33 (2006.01); G01N 21/05 (2006.01); G01N 21/85 (2006.01); G01N 30/74 (2006.01); G01N 21/31 (2006.01); G01N 30/02 (2006.01)
CPC G01N 21/33 (2013.01) [G01N 21/05 (2013.01); G01N 21/8507 (2013.01); G01N 30/74 (2013.01); G01N 2021/3125 (2013.01); G01N 2030/027 (2013.01); G01N 2201/0624 (2013.01); G01N 2201/0625 (2013.01); G01N 2201/0691 (2013.01); G01N 2201/127 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A method for measuring the absorbance of light of a substance in a solution in a measuring cell, said method comprising the steps of:
transmitting a first light beam from a light source towards a beam splitter;
dividing the first light beam into a signal light ray and a reference light ray by the beam splitter;
modulating the signal light ray;
modulating the reference light ray;
providing the measuring cell such that the signal light ray passes through the measuring cell;
detecting a signal in a detector, which signal is the combined signal intensity of the signal light ray and the reference light ray detected by the detector;
performing synchronous detection of the detected signal in order to reconstruct the intensities of the signal light ray and the reference light ray from the combined signal detected by the detector, said synchronous detection being based on the modulation performed to the signal light ray and the reference light ray in order to enable measurement of absorbance changes over time in a flowing sample.