| CPC C23C 2/04 (2013.01) | 5 Claims |

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1. A Raman spectrometry test specimen comprises a metallic particle-deposition substrate, the metallic particle-deposition substrate comprising:
a metal substrate comprising a first metallic material;
a plurality of metallic particles on a surface of the metal substrate in a single particulate layer, the metallic particles are nanoparticles, wherein the metallic particles comprise a second metallic material different from the first metallic material, wherein at least 50% of the metallic particles do not contact or overlap and are attached to at least 50% of the surface of the metal substrate; and
an alloy layer at an interface between the metal substrate and the metallic particles, the alloy layer having a thickness less than 50 nm;
wherein a material of the alloy layer is a combination of the first metallic material and the second metallic material; and
wherein at least 50% of the metallic particles have each metallic particle in a distance at a range of between 0.5 nm and 100 nm between each individual adjacent metallic particle.
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