	US 12,275,999 B2
	Polynucleotides for the amplification and detection of chlamydia trachomatis
Matthew B. Lee, San Jose, CA (US); and Hedia Maamar, San Jose, CA (US)
Assigned to Talis Biomedical Corporation, Chicago, IL (US)
Appl. No. 16/349,185
Filed by Talis Biomedical Corporation, Menlo Park, CA (US)
PCT Filed Nov. 13, 2017, PCT No. PCT/US2017/061402 § 371(c)(1), (2) Date May 10, 2019, PCT Pub. No. WO2018/089942, PCT Pub. Date May 17, 2018.
Claims priority of provisional application 62/420,488, filed on Nov. 10, 2016.
Prior Publication US 2019/0284617 A1, Sep. 19, 2019
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/68 (2018.01); C07H 21/00 (2006.01); C07H 21/04 (2006.01); C12Q 1/6844 (2018.01); C12Q 1/689 (2018.01)

CPC C12Q 1/689 (2013.01) [C07H 21/00 (2013.01); C07H 21/04 (2013.01); C12Q 1/6844 (2013.01)]

11 Claims

1. A method of detecting Chlamydia trachomatis in a test sample, the method comprising:

(a) extracting nucleic acid from the test sample;

(b) amplifying a target sequence by reacting the nucleic acid extracted in step (a) with a reaction mixture comprising a strand displacement DNA polymerase and a sequence-specific primer set comprising Set-1, wherein Set-1 comprises SEQ ID NO: 3 and SEQ ID NO: 4; and

(c) detecting the presence or absence of an amplified product of step (b); wherein the presence of said amplification product is indicative of the presence of Chlamydia trachomatis in the test sample.