| CPC C12Q 1/6886 (2013.01) [C12Q 1/686 (2013.01); G01N 21/6486 (2013.01); C12Q 2600/158 (2013.01); C12Q 2600/178 (2013.01)] | 15 Claims |
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1. A system, comprising:
a droplet apparatus, in a first configuration, capable of forming a plurality of discrete sample portions of a sample comprising a target nucleic acid, each of the plurality of discrete sample portions comprising a portion of the sample, a reference dye, and a reaction mixture, wherein the plurality of discrete sample portions comprises discrete sample portions of a plurality of sizes;
an amplification apparatus configured to amplify the plurality of discrete sample portions to form at least one discrete processed sample portion containing nucleic acid amplification reaction product of the target nucleic acid;
a detection apparatus configured to:
detect a fluorescence signal from the at least one of the plurality of discrete processed sample portions in response to a presence of the at least one target nucleic acid and a light scattering property, and
detect a real-time fluorescence signal from the at least one of the plurality of discrete sample portions to determine a Cq value;
an optical imager configured to produce an image of the plurality of discrete processed sample portions; and
a processor configured to:
determine the respective volumes of the plurality of discrete processed sample portions, from the image, by measuring an optical property of each discrete processed sample portion to determine a dimension of each discrete processed sample portion, and
determine an amount of the target nucleic acid based on the number of discrete processed sample portions containing the target nucleic acid therein, and the Cq value.
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