US 12,275,993 B2
Analysis of nucleic acid sequences
Solongo B. Ziraldo, Pleasanton, CA (US); Geoffrey McDermott, Livermore, CA (US); and Shea Thompson Lance, Livermore, CA (US)
Assigned to 10X GENOMICS, INC., Pleasanton, CA (US)
Filed by 10x Genomics, Inc., Pleasanton, CA (US)
Filed on Oct. 28, 2024, as Appl. No. 18/929,514.
Application 18/929,514 is a continuation in part of application No. 17/397,775, filed on Aug. 9, 2021.
Application 17/397,775 is a continuation of application No. PCT/US2020/017785, filed on Feb. 11, 2020.
Claims priority of provisional application 62/934,256, filed on Nov. 12, 2019.
Claims priority of provisional application 62/804,633, filed on Feb. 12, 2019.
Prior Publication US 2025/0051847 A1, Feb. 13, 2025
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6883 (2018.01); C12Q 1/6827 (2018.01); G16B 30/00 (2019.01); G16B 30/10 (2019.01); G16B 30/20 (2019.01)
CPC C12Q 1/6883 (2013.01) [C12Q 1/6827 (2013.01); G16B 30/00 (2019.02); G16B 30/10 (2019.02); G16B 30/20 (2019.02); C12Q 2535/122 (2013.01); C12Q 2600/156 (2013.01)] 30 Claims
 
1. A method comprising:
(a) providing:
a first emulsion comprising a first droplet population, wherein droplets of the first droplet population comprise a lysis reagent, and
a second emulsion comprising a second droplet population, wherein a droplet of the second droplet population comprises i) a cell or a nucleus, and ii) a plurality of nucleic acid barcode molecules;
(b) subjecting the first emulsion and second emulsion to conditions sufficient to transfer the lysis reagent to the second droplet population via micelles comprising the lysis reagent; and
(c) lysing the cell or the nucleus within the droplet of the second droplet population with the lysis reagent.