	US 12,275,979 B2
	Gene-modified microorganism for producing 3-hydroxyadipic acid, alpha-hydromuconic acid, and/or adipic acid, and production method for said chemical products
Kyohei Isobe, Kamakura (JP); Kenji Kawamura, Kamakura (JP); and Katsushige Yamada, Kamakura (JP)
Assigned to TORAY INDUSTRIES, INC., Tokyo (JP)
Appl. No. 16/766,979
Filed by TORAY INDUSTRIES, INC., Tokyo (JP)
PCT Filed Nov. 29, 2018, PCT No. PCT/JP2018/044080 § 371(c)(1), (2) Date May 26, 2020, PCT Pub. No. WO2019/107516, PCT Pub. Date Jun. 6, 2019.
Claims priority of application No. 2017-230407 (JP), filed on Nov. 30, 2017.
Prior Publication US 2020/0291435 A1, Sep. 17, 2020
Int. Cl. C12N 1/21 (2006.01); C12P 7/42 (2006.01); C12P 7/44 (2006.01)

CPC C12P 7/42 (2013.01) [C12P 7/44 (2013.01)]

8 Claims

1. A genetically modified non-naturally occurring microorganism comprising a genetic modification in which a nucleic acid encoding a polypeptide is introduced or expression of the polypeptide is enhanced as compared to an otherwise identical microorganism without the genetic modification, so as to increase an amount of 3-hydroxy adipic acid, α-hydromuconic acid or adipic acid produced by said non-naturally occurring microorganism compared to an otherwise identical microorganism without the genetic modification;

wherein the polypeptide is composed of an amino acid sequence selected from the group consisting of SEQ ID NOs: 1 to 6 and 213; and

wherein an amount of 3-hydroxy adipic acid, α-hydromuconic acid or adipic acid produced by said non-naturally occurring microorganism is increased compared to an otherwise identical microorganism without the genetic modification.