	US 12,275,976 B2
	Phloroglucinol-resistant cell, in particular yeast
Odile Ramaen, Ablis (FR); Vincent Lafaquiere, Clermont-Ferrand (FR); and Dominique Louis, Forges les Bains (FR)
Assigned to COMPAGNIE GENERALE DES ETABLISSEMENTS MICHELIN, Clermont-Ferrand (FR)
Appl. No. 17/428,827
Filed by COMPAGNIE GENERALE DES ETABLISSEMENTS MICHELIN, Clermont-Ferrand (FR)
PCT Filed Feb. 5, 2020, PCT No. PCT/FR2020/050189 § 371(c)(1), (2) Date Aug. 5, 2021, PCT Pub. No. WO2020/161436, PCT Pub. Date Aug. 13, 2020.
Claims priority of application No. 1901115 (FR), filed on Feb. 5, 2019.
Prior Publication US 2022/0098626 A1, Mar. 31, 2022
Int. Cl. C12N 9/10 (2006.01); C12N 15/52 (2006.01); C12P 7/22 (2006.01)

CPC C12P 7/22 (2013.01) [C12N 9/1029 (2013.01); C12N 15/52 (2013.01)]

17 Claims

1. An isolated living microorganism that is phloroglucinol resistant, wherein the isolated living microorganism withstands a phloroglucinol concentration, in a culture medium of the isolated living microorganism, of greater than or equal to 1 g·l⁻¹, and

wherein the isolated living microorganism overexpresses at least one membrane transporter belonging to the PDR subfamily or to the MFS transporter family, or at least one transcription factor which controls the expression of said membrane transporter, said membrane transporter or said transcription factor being selected from SNQ2, STE6, PDR3, AQR1, DTR1,FLR1, QDR1, YHK8 and ATR1.