US 12,275,972 B2
Microorganism producing purine nucleotide and method of producing purine nucleotide using the same
Ji Hyun Lee, Seoul (KR); Sojung Park, Seoul (KR); Hee Su Kwon, Seoul (KR); Dae Young Kim, Seoul (KR); and Ji Hye Lee, Seoul (KR)
Assigned to CJ CHEILJEDANG CORPORATION, Seoul (KR)
Appl. No. 17/296,375
Filed by CJ CHEILJEDANG CORPORATION, Seoul (KR)
PCT Filed Feb. 19, 2021, PCT No. PCT/KR2021/002150
§ 371(c)(1), (2) Date May 24, 2021,
PCT Pub. No. WO2021/167414, PCT Pub. Date Aug. 26, 2021.
Claims priority of application No. 10-2020-0021383 (KR), filed on Feb. 21, 2020.
Prior Publication US 2022/0380821 A1, Dec. 1, 2022
Int. Cl. C12P 19/32 (2006.01); C07K 14/34 (2006.01); C12N 15/77 (2006.01)
CPC C12P 19/32 (2013.01) [C07K 14/34 (2013.01); C12N 15/77 (2013.01); C12N 2800/101 (2013.01)] 5 Claims
 
1. A method of producing a purine nucleotide, the method comprising:
the step of culturing, in a medium, a microorganism of the genus Corynebacterium having an increased purine nucleotide productivity compared to an unmodified microorganism, wherein the microorganism of the genus Corynebacterium
(a) is modified such that an activity of a phosphate importer system encoded by pstSCAB operon is enhanced in comparison with an endogenous activity thereof, and
(b) is Corynebacterium stationis, and
the step of recovering the purine nucleotide from the cultured medium or microorganism.