US 12,275,965 B2
Nuclease domain and use thereof
Takashi Yamamoto, Higashi-Hiroshima (JP); Tetsushi Sakuma, Higashi-Hiroshima (JP); and Masakazu Saito, Higashi-Hiroshima (JP)
Assigned to HIROSHIMA UNIVERSITY, Higashi-Hiroshima (JP)
Appl. No. 17/271,481
Filed by HIROSHIMA UNIVERSITY, Higashi-Hiroshima (JP)
PCT Filed Aug. 23, 2019, PCT No. PCT/JP2019/033045
§ 371(c)(1), (2) Date Feb. 25, 2021,
PCT Pub. No. WO2020/045281, PCT Pub. Date Mar. 5, 2020.
Claims priority of application No. 2018-158710 (JP), filed on Aug. 27, 2018.
Prior Publication US 2021/0332339 A1, Oct. 28, 2021
Int. Cl. C12N 9/22 (2006.01); C12N 15/63 (2006.01); C12N 15/90 (2006.01)
CPC C12N 9/22 (2013.01) [C12N 15/63 (2013.01); C12N 15/907 (2013.01); C12N 2800/80 (2013.01)] 10 Claims
 
1. A combination of two artificial nucleic acid-cleaving enzymes comprising:
a first artificial nucleic acid-cleaving enzyme comprising a nuclease domain which is a polypeptide comprising the amino acid sequence set forth in SEQ ID NO 69 and a nucleic acid-binding domain, and
a second artificial nucleic acid-cleaving enzyme comprising a nuclease domain which is a polypeptide comprising the amino acid sequence set forth in SEQ ID NO 70 and a nucleic acid-binding domain,
wherein the nucleic acid-binding domain of the first artificial nucleic acid-cleaving enzyme and of the second artificial nucleic acid-cleaving enzyme is a zinc finger.