US 12,275,958 B2
Gut organoid and method for producing the same
Takuya Okazaki, Tokyo (JP); Akihiro Umezawa, Tokyo (JP); Hidenori Akutsu, Tokyo (JP); and Hajime Uchida, Tokyo (JP)
Assigned to DAI NIPPON PRINTING CO., LTD., Tokyo (JP); and NATIONAL CENTER FOR CHILD HEALTH AND DEVELOPMENT, Tokyo (JP)
Filed by DAI NIPPON PRINTING CO., LTD., Tokyo (JP); and NATIONAL CENTER FOR CHILD HEALTH AND DEVELOPMENT, Tokyo (JP)
Filed on Dec. 13, 2019, as Appl. No. 16/713,808.
Application 16/713,808 is a continuation of application No. PCT/JP2018/013950, filed on Mar. 30, 2018.
Claims priority of application No. 2017-115952 (JP), filed on Jun. 13, 2017.
Prior Publication US 2020/0102543 A1, Apr. 2, 2020
Int. Cl. C12N 5/071 (2010.01); G01N 33/50 (2006.01)
CPC C12N 5/0697 (2013.01) [C12N 5/0679 (2013.01); G01N 33/5082 (2013.01); C12N 2506/02 (2013.01); C12N 2506/45 (2013.01); C12N 2513/00 (2013.01)] 32 Claims
 
1. An in vitro gut organoid comprising:
a tissue structure that encloses a cavity, wherein:
the cavity is a closed cavity that is able to hold liquid;
the tissue structure comprises endodermal cells, mesodermal cells, and ectodermal cells; and
the endodermal cells comprise intestinal epithelial cells that are at least part of the outermost surface of the tissue structure.