	US 12,275,954 B2
	Method of identifying l/m-opsin and CRX positive cone photoreceptor cells based on specific biomarkers
Jane Sowden, London (GB); Jorn Lakowski, London (GB); Robin Ali, London (GB); Emily Welby, London (GB); and Anai Gonzalez Cordero, London (GB)
Assigned to UCL BUSINESS Ltd., London (GB)
Appl. No. 16/488,380
Filed by UCL BUSINESS Ltd., London (GB)
PCT Filed Feb. 21, 2018, PCT No. PCT/GB2018/050452 § 371(c)(1), (2) Date Aug. 23, 2019, PCT Pub. No. WO2018/154295, PCT Pub. Date Aug. 30, 2018.
Claims priority of application No. 1703058 (GB), filed on Feb. 24, 2017.
Prior Publication US 2020/0063095 A1, Feb. 27, 2020
Int. Cl. C12N 5/07 (2010.01); A61K 9/00 (2006.01); A61K 35/30 (2015.01); C12N 5/071 (2010.01); C12N 5/0735 (2010.01); C12N 5/074 (2010.01); C12N 5/079 (2010.01); C12N 5/0793 (2010.01); C12N 5/0797 (2010.01); G01N 33/53 (2006.01); G01N 33/569 (2006.01); G01N 33/68 (2006.01)

CPC C12N 5/062 (2013.01) [A61K 9/0048 (2013.01); A61K 35/30 (2013.01); G01N 33/56966 (2013.01); G01N 33/68 (2013.01); C12N 2506/02 (2013.01); C12N 2506/45 (2013.01)]

7 Claims

1. A method of identifying L/M-Opsin and CRX positive cone photoreceptor cells in a population of cells, comprising: a) detecting and determining whether or not cells in the population express CD29 or CD15-SSEA1 on cell surface; b) detecting and determining whether or not cells in the population express at least two of CD26, CD133 and CD 147 on the cell surface; and c) identifying cells as L/M-Opsin and CRX positive cone photoreceptor cells based on presence of L/M-Opsin and CRX, absence of CD29 or CD15-SSEA1, and presence of at least two of CD26, CD133 and CD147, and wherein the cells are L/M-Opsin and CRX positive, CD29 or CD15-SSEA1 negative and positive for at least two of CD26, CD133 and CD147.