	US 12,275,945 B2
	Methods for improving photosynthetic organisms
Nicholas John Roberts, Feilding (NZ); Somrutai Winichayakul, Palmerston North (NZ); Zacharia D'Arcy Beechey-Gradwell, Palmerston North (NZ); and Luke James Cooney, Waitarere Beach (NZ)
Assigned to AGRESEARCH LIMITED, Canterbury (NZ)
Appl. No. 17/755,168
Filed by AGRESEARCH LIMITED, Canterbury (NZ)
PCT Filed Oct. 22, 2020, PCT No. PCT/IB2020/059915 § 371(c)(1), (2) Date Apr. 22, 2022, PCT Pub. No. WO2021/079297, PCT Pub. Date Apr. 29, 2021.
Claims priority of application No. 2019904013 (AU), filed on Oct. 25, 2019.
Prior Publication US 2022/0290174 A1, Sep. 15, 2022
Int. Cl. C12N 15/82 (2006.01); C07K 14/415 (2006.01); C12N 9/10 (2006.01)

CPC C12N 15/8269 (2013.01) [C07K 14/415 (2013.01); C12N 9/1029 (2013.01); C12N 15/8273 (2013.01)]

10 Claims

1. A method for reducing water-soluble carbohydrates (WSC) in a photosynthetic cell or plant, the method comprising the steps of:

(a) genetically modifying the photosynthetic cell or plant to express:

a modified oleosin including at least one artificially introduced cysteine, wherein

(i) the cysteine is introduced into at least one of the N-terminal hydrophilic region of the oleosin and the C-terminal hydrophilic region of the oleosin; and

(ii) at least one diacylglycerol acyltransferase-1 (DGAT 1); and

(b) measuring WSC amounts in the photosynthetic cell or plant, and

(c) selecting a photosynthetic cell or plant with reduced WSC relative to that in a control photosynthetic cell or plant.