US 12,275,943 B2
Method for increasing intracellular heme content of Escherichia coli
Lei Tang, Wuxi (CN); Mei Pan, Wuxi (CN); and Shuangxin Liu, Wuxi (CN)
Assigned to Jiangnan University, Wuxi (CN)
Filed by Jiangnan University, Wuxi (CN)
Filed on Sep. 1, 2021, as Appl. No. 17/463,619.
Application 17/463,619 is a continuation of application No. PCT/CN2020/118317, filed on Sep. 28, 2020.
Claims priority of application No. 202010651132.6 (CN), filed on Jul. 8, 2020.
Prior Publication US 2021/0395755 A1, Dec. 23, 2021
Int. Cl. C12N 1/20 (2006.01); C12N 9/02 (2006.01); C12N 9/10 (2006.01); C12N 15/70 (2006.01)
CPC C12N 15/70 (2013.01) [C12N 1/20 (2013.01); C12N 9/0008 (2013.01); C12N 9/1085 (2013.01); C12Y 102/0107 (2013.01); C12Y 205/01054 (2013.01)] 7 Claims
 
1. A recombinant strain of Escherichia coli(E. Coli) comprising two or more of the following genetic mutations,
(a) a knocked out or silenced mscS gene encoding a mechanically sensitive ion channel protein that is set forth in SEQ ID NO:1,
(b) a knocked out or silenced aroG gene encoding a 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase that is set forth in SEQ ID NO:2, and
(c) an overexpressed hemA gene encoding a glutamyl-tRNA reductase that is set forth in SEQ ID NO:3.