US 12,275,939 B2
Modifying the specificity of plant non-coding RNA molecules for silencing gene expression
Eyal Maori, Cambridge (GB); Yaron Galanty, Cambridge (GB); Cristina Pignocchi, Norwich (GB); Angela Chaparro Garcia, Norwich (GB); and Ofir Meir, Norwich (GB)
Assigned to Tropic Biosciences UK Limited, Norwich (GB)
Filed by Tropic Biosciences UK Limited, Norwich (GB)
Filed on Jan. 10, 2023, as Appl. No. 18/152,722.
Application 18/152,722 is a continuation of application No. 16/648,748, granted, now 11,555,199, previously published as PCT/IB2018/057160, filed on Sep. 18, 2018.
Claims priority of application No. 1715113 (GB), filed on Sep. 19, 2017; application No. 1715116 (GB), filed on Sep. 19, 2017; and application No. 1719516 (GB), filed on Nov. 23, 2017.
Prior Publication US 2023/0340512 A1, Oct. 26, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/113 (2010.01); C12N 9/22 (2006.01); C12N 15/82 (2006.01); C12N 15/85 (2006.01); C12N 15/90 (2006.01)
CPC C12N 15/113 (2013.01) [C12N 9/22 (2013.01); C12N 15/8213 (2013.01); C12N 15/8279 (2013.01); C12N 15/85 (2013.01); C12N 15/902 (2013.01); C12N 15/8218 (2013.01); C12N 2310/11 (2013.01); C12N 2310/141 (2013.01); C12N 2310/20 (2017.05)] 18 Claims
 
1. A method of modifying a DNA sequence encoding or processed into an RNA silencing molecule with a silencing specificity towards a first target RNA in a plant cell, the method comprising introducing into the plant cell a DNA editing agent and a donor oligonucleotide to generate a desired modification in the DNA sequence, thereby modifying the DNA sequence encoding the RNA silencing molecule, wherein the modification redirects the silencing specificity of said RNA silencing molecule towards a second target RNA, said first target RNA and said second target RNA being distinct.