	US 12,275,933 B2
	Composition of photoautotrophic microorganisms and chemoheterotrophic microorganisms in a biofilm
Rohan Karande, Leipzig (DE); Ingeborg Heuschkel, Leipzig (DE); Katja Bühler, Leipzig (DE); Anna Hoschek, Leipzig (DE); and Andreas Schmid, Leipzig (DE)
Assigned to Helmholtz-Zentrum für Umweltforschung GmbH—UFZ, Leipzig (DE)
Appl. No. 17/276,075
Filed by Helmholtz-Zentrum für Umweltforschung GmbH—UFZ, Leipzig (DE)
PCT Filed Sep. 13, 2018, PCT No. PCT/EP2018/074746 § 371(c)(1), (2) Date Mar. 12, 2021, PCT Pub. No. WO2020/052762, PCT Pub. Date Mar. 19, 2020.
Prior Publication US 2022/0049210 A1, Feb. 17, 2022
Int. Cl. C12N 1/20 (2006.01); C12M 1/00 (2006.01); C12M 1/12 (2006.01); C12P 7/22 (2006.01); C12P 17/08 (2006.01); C12P 39/00 (2006.01)

CPC C12N 1/20 (2013.01) [C12M 21/02 (2013.01); C12M 23/06 (2013.01); C12M 23/22 (2013.01); C12M 25/06 (2013.01); C12P 7/22 (2013.01); C12P 17/08 (2013.01); C12P 39/00 (2013.01)]

14 Claims

1. A biofilm composition having a cell density of 30 grams of cell dry weight per liter or more, comprising: photoautotrophic Synechocystis which produce oxygen by photosynthetic water oxidation; chemoheterotrophic Pseudomonas taiwanensis which respire oxygen; and components which are secreted by the Synechocystis and/or the Pseudomonas taiwanensis, wherein the Synechocystis and/or the Pseudomonas taiwanensis are genetically modified to express cytochrome P450 for the conversion of cyclohexane to cyclohexanol, or genetically modified to express cyclohexanone monooxygenase for the conversion of cyclohexanone to ε-caprolactone.