| CPC C12M 47/04 (2013.01) [C12M 41/48 (2013.01); G01N 27/4145 (2013.01); G06V 20/693 (2022.01)] | 7 Claims |
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1. A cell isolation device, comprising:
a cell manipulation panel, comprising:
a first substrate and a second substrate spaced apart, forming a cell gap therebetween, wherein the cell gap is configured to be provided with a fluid medium having a plurality of cells therein, and the cells comprise wanted cells and unwanted cells;
an insulating layer disposed on the first substrate, forming a plurality of vias penetrating therethrough;
a pixel array disposed between the insulating layer and the first substrate, defining a plurality of pixels, wherein each of the pixels has a first thin film transistor (TFT) and corresponds to a corresponding via of the vias, the first TFT comprises a first gate electrode, a first electrode and a second electrode, the first gate electrode is provided with a first gate signal, the first gate signal is switched between an ON signal and an OFF signal, the first TFT is configured to be turned on by providing the ON signal as the first gate signal and to be turned off by providing the OFF signal as the first gate signal, the first electrode is configured to be grounded, and the second electrode is located corresponding to the corresponding via such that the second electrode is partially exposed to the fluid medium in the cell gap through the corresponding via;
a common electrode disposed on the second substrate, being provided with a common voltage VCOM; and
a plurality of bottom electrodes disposed between the insulating layer and the first substrate, wherein each of the bottom electrodes is configured to be provided with a bottom voltage in an operational mode;
an imaging device, disposed on a side of the cell manipulation panel adjacent to one of the first substrate and the second substrate, configured to capture an image of the pixels having cells being captured in the vias; and
a controller, communicatively connected to the imaging device and the cell manipulation panel, wherein the controller is configured to:
control a first gate driver to provide either the ON signal or the OFF signal as the first gate signal to each of the pixels;
control a second gate driver to provide a second gate signal to control the first electrode of each of the pixels to be grounded or not to be grounded;
control the cell manipulation panel to capture the cells in the pixels by controlling the first gate driver to provide the OFF signal as the first gate signal to all of the pixels and controlling the second gate driver to provide the second gate signal to control the first electrode of each of the pixels not to be grounded, wherein each of the pixels captures a corresponding one of the cells in the corresponding via from the fluid medium by a dielectrophoresis (DEP) force;
receive the image obtained by the imaging device;
analyze the image and determine the pixels having the wanted cells being captured therein and the pixels having the unwanted cells being captured therein; and
in response to determining the pixels having the unwanted cells being captured therein, control the cell manipulation panel to release the unwanted cells to the fluid medium by:
controlling the first gate driver to provide the ON signal as the first gate signal to each of the pixels having the unwanted cells being captured therein, such that in each of the pixels having the unwanted cells being captured therein, the first TFT is turned on, and the first electrode and the second electrode are electrically connected by the first TFT being turned on; and
controlling the second gate driver to provide the second gate signal to control the first electrode and the second electrode of each of the pixels having the unwanted cells being captured therein to be grounded.
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