US 12,275,929 B2
Cell expansion system
Yuguo Lei, Lincoln, NE (US); and Hendrik Viljoen, Adams, NE (US)
Assigned to NUtech Ventures, Lincoln, NE (US)
Appl. No. 16/981,050
Filed by NUtech Ventures, Lincoln, NE (US)
PCT Filed Mar. 15, 2019, PCT No. PCT/US2019/022594
§ 371(c)(1), (2) Date Sep. 15, 2020,
PCT Pub. No. WO2019/178549, PCT Pub. Date Sep. 19, 2019.
Claims priority of provisional application 62/643,894, filed on Mar. 16, 2018.
Prior Publication US 2021/0017485 A1, Jan. 21, 2021
Int. Cl. C12M 1/00 (2006.01); C12M 1/12 (2006.01); C12M 1/34 (2006.01); C12N 5/00 (2006.01)
CPC C12M 41/46 (2013.01) [C12M 23/20 (2013.01); C12M 25/14 (2013.01); C12M 25/16 (2013.01); C12N 5/0068 (2013.01); C12N 2500/14 (2013.01); C12N 2533/40 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A cell expansion system for expanding cells, the system comprising:
a cap connected to a tubular housing defining an internal space, the cap comprising: an extruder comprising at least a first inlet and at least a second inlet, the extruder disposed within the internal space defined by the cap and the tubular housing such that distal ends of the first inlet and the second inlet are connected to the cap at respective openings in the cap, the first inlet operable for introducing a cell solution into the cap, the at least second inlet operable for introducing a hydrogel-forming solution into the cap; and
the tubular housing in fluid connection with the extruder, wherein the tubular housing comprises a cell compatible buffer.