	US 12,275,924 B2
	Method and device for detecting circulating tumor cell
Qihui Shi, Shanghai (CN); Shun Lu, Shanghai (CN); Yuliang Deng, Shanghai (CN); and Yin Tang, Shanghai (CN)
Assigned to SHANGHAI JIAOTONG UNIVERSITY, Shanghai (CN)
Filed by SHANGHAI JIAOTONG UNIVERSITY, Shanghai (CN)
Filed on Jul. 16, 2021, as Appl. No. 17/377,956.
Application 17/377,956 is a division of application No. 15/749,126, granted, now 11,098,274, previously published as PCT/CN2016/080789, filed on Apr. 29, 2016.
Claims priority of application No. 201510466462.7 (CN), filed on Jul. 31, 2015.
Prior Publication US 2024/0228921 A1, Jul. 11, 2024
Int. Cl. C12M 1/34 (2006.01); C12Q 1/02 (2006.01); G01N 33/569 (2006.01); G01N 33/574 (2006.01)

CPC C12M 1/34 (2013.01) [C12Q 1/02 (2013.01); G01N 33/569 (2013.01); G01N 33/57492 (2013.01); G01N 2333/70589 (2013.01)]

10 Claims

1. A method for detecting metabolic activity of a circulating tumor cell, which comprises the steps of:

(a) enriching and isolating a circulating tumor cell or CTC from a peripheral blood sample, thereby obtaining the isolated circulating tumor cell;

(b) placing the circulating tumor cell isolated in the above step in a microwell array chip, so that there is at most one circulating tumor cell in each microwell of the microwell array chip;

(c) resuscitating the circulating tumor cell in the above step, thereby obtaining the resuscitated circulating tumor cell;

(d) culturing the resuscitated circulating tumor cell in the presence of a glucose analogue carrying a detectable label; and

(e) detecting uptake of the glucose analogue carrying the detectable label by the circulating tumor cell to qualitatively or quantitatively determine metabolic activity and/or malignancy degree of the circulating tumor cell; wherein the glucose analogue is 2-NBDG.