US 12,275,920 B2
Compositions and methods for clarification of biological materials
Sunghee Lee Dimauro, Stamford, CT (US)
Filed by Sunghee Lee Dimauro, Stamford, CT (US)
Filed on Jun. 27, 2021, as Appl. No. 17/359,609.
Claims priority of provisional application 63/045,171, filed on Jun. 28, 2020.
Prior Publication US 2021/0325282 A1, Oct. 21, 2021
Int. Cl. C11D 3/16 (2006.01); C11D 1/66 (2006.01); G01N 21/41 (2006.01); G01N 33/483 (2006.01)
CPC C11D 3/168 (2013.01) [G01N 33/4833 (2013.01); C11D 1/66 (2013.01); G01N 21/41 (2013.01)] 16 Claims
OG exemplary drawing
 
9. A method for a clearing treatment for increasing the transparency of a biological material, the method comprising:
permeating, into an isolated biological material, a clearing composition comprising an aqueous solution of a polyoxometallate salt present in an amount of at least about 50 wt %, the clearing composition comprising a refractive index of greater than about 1.40 at a visible light wavelength, the clearing composition permeating into the isolated biological material so that the isolated biological material is increased in transparency,
wherein the isolated biological material comprises tissue or organ derived from a multicellular animal; and
after permeating, performing an observation step of the isolated biological material with an optical microscopy technique.