US 12,275,801 B2
Isolation of adult multipotential cells by tissue non-specific alkaline phosphatase
Stan Gronthos, Adelaide (AU); Andrew Christopher William Zannettino, Highbury (AU); and John Paul Simmons, Kew (AU)
Assigned to MESOBLAST, INC., New York, NY (US)
Filed by Stan Gronthos, Adelaide (AU); Andrew Christopher William Zannettino, Highbury (AU); and John Paul Simmons, Kew (AU)
Filed on May 5, 2020, as Appl. No. 16/867,252.
Application 15/167,934 is a division of application No. 14/621,470, filed on Feb. 13, 2015, abandoned.
Application 14/621,470 is a division of application No. 13/736,790, filed on Jan. 8, 2013, abandoned.
Application 16/867,252 is a continuation of application No. 15/651,469, filed on Jul. 17, 2017, abandoned.
Application 15/651,469 is a continuation of application No. 15/167,934, filed on May 27, 2016, granted, now 9,783,617, issued on Oct. 10, 2017.
Application 13/736,790 is a continuation of application No. 11/918,593, granted, now 8,367,405, issued on Feb. 5, 2013, previously published as PCT/AU2006/000494, filed on Apr. 12, 2006.
Claims priority of provisional application 60/670,250, filed on Apr. 12, 2005.
Prior Publication US 2020/0332023 A1, Oct. 22, 2020
Int. Cl. C12N 5/0775 (2010.01); A61K 35/12 (2015.01); C07K 16/40 (2006.01); C12N 5/00 (2006.01); C12N 5/16 (2006.01); C12Q 1/42 (2006.01); G01N 33/577 (2006.01); G01N 33/68 (2006.01)
CPC C07K 16/40 (2013.01) [C12N 5/0081 (2013.01); C12N 5/0663 (2013.01); C12N 5/163 (2013.01); C12Q 1/42 (2013.01); G01N 33/577 (2013.01); G01N 33/68 (2013.01); A61K 2035/124 (2013.01); C07K 2317/76 (2013.01); C12N 2533/56 (2013.01); G01N 2333/916 (2013.01)] 20 Claims
 
1. A cell population comprising multipotential cells enriched for TNAP+multipotential cells, wherein at least 4% of the total cell population are TNAP+, STRO-1+multipotential cells capable of being cultured in vitro to produce adipocytes, osteocytes and chondrocytes and wherein the TNAP+STRO-1+multipotential cells include a TNAP+marker which binds to the STRO-3 antibody produced by the hybridoma cell line deposited under ATCC Accession Number PTA-7282.