	US 12,275,761 B2
	Mutant CsgG pores
Stefan Howorka, London (GB); Han Remaut, Roosbeek (BE); Lakmal Jayasinghe, Oxford (GB); Elizabeth Jayne Wallace, Oxford (GB); James Clarke, Oxford (GB); Richard George Hambley, Oxford (GB); and Jonathan Bankes Pugh, Oxford (GB)
Assigned to VIB VZW, Ghent (BE); Vrije Universiteit Brussel, Brussels (BE); and Oxford Nanopore Technologies PLC, Oxford (GB)
Filed by Oxford Nanopore Technologies PLC, Oxford (GB); VIB VZW, Ghent (BE); and Vrije Universiteit Brussel, Brussels (BE)
Filed on Mar. 8, 2021, as Appl. No. 17/194,821.
Application 17/194,821 is a continuation of application No. 16/522,591, filed on Jul. 25, 2019, granted, now 11,034,734.
Application 16/522,591 is a continuation of application No. 15/507,947, granted, now 10,400,014, previously published as PCT/EP2015/069965, filed on Sep. 1, 2015.
Claims priority of application No. 1422079 (GB), filed on Dec. 11, 2014; application No. 1506754 (GB), filed on Apr. 21, 2015; and application No. 1508287 (GB), filed on May 14, 2015.
Prior Publication US 2021/0292376 A1, Sep. 23, 2021
Int. Cl. C07K 14/245 (2006.01); C12Q 1/68 (2018.01); C12Q 1/6869 (2018.01); C12Q 1/6874 (2018.01); G01N 27/447 (2006.01)

CPC C07K 14/245 (2013.01) [C12Q 1/6869 (2013.01); C12Q 1/6874 (2013.01); G01N 27/447 (2013.01); C07K 2319/22 (2013.01); C12Q 1/68 (2013.01); C12Q 2565/631 (2013.01)]

5 Claims

1. A method measuring a target analyte, comprising:

(a) contacting the target analyte with a transmembrane pore comprising at least one CsgG monomer such that the target analyte moves through the pore; and

(b) taking one or more electrical measurements as the target analyte moves through the pore.