	US 12,274,993 B2
	Systems and methods for affecting interactions of electromagnetic radiation with janus droplets for sensitive detection of species
Timothy Manning Swager, Newton, MA (US); Jie Li, Waltham, MA (US); Suchol Savagatrup, Cambridge, MA (US); Zachary P. Nelson, Cambridge, MA (US); Kosuke Yoshinaga, Cambridge, MA (US); and Cassandra Zentner, Brighton, MA (US)
Assigned to Massachusetts Institute of Technology, Cambridge, MA (US)
Filed by Massachusetts Institute of Technology, Cambridge, MA (US)
Filed on Jul. 17, 2020, as Appl. No. 16/932,392.
Claims priority of provisional application 62/902,367, filed on Sep. 18, 2019.
Prior Publication US 2021/0080456 A1, Mar. 18, 2021
Int. Cl. B01L 1/00 (2006.01); B01J 13/04 (2006.01); B01J 13/14 (2006.01); C01B 33/145 (2006.01); G01N 33/543 (2006.01)

CPC B01J 13/14 (2013.01) [B01J 13/046 (2013.01); C01B 33/145 (2013.01); G01N 33/5432 (2013.01)]

11 Claims

1. A system comprising:

a plurality of Janus droplets comprising an interface between a first phase and a second phase of the droplets, the second phase immiscible with the first phase; and

a binding moiety to a biological analyte associated with the plurality of Janus droplets,

wherein the first phase comprises an emissive species that interacts with electromagnetic radiation in a detectable manner,

wherein the second phase comprises a blocking species that at least partially absorbs and/or reflects electromagnetic radiation such that the blocking species at least partially blocks the passage of electromagnetic radiation that interacts with the first phase and/or at least partially blocks the passage of electromagnetic radiation emitted by the first phase,

wherein the plurality of Janus droplets have a first orientation in the absence of the biological analyte and a second orientation upon binding of the binding moiety to the biological analyte, such that the electromagnetic radiation emitted or transmitted in the second orientation is less than in the first orientation.