US 12,274,732 B2
Anti-pathogen composition and methods of use thereof
Shaohua Guan, Frisco, TX (US); Shashi Shankar Rajbanshi, Frisco, TX (US); Curtis Brian Hill, Little Elm, TX (US); Shi Qiu, Oxford, MS (US); and Xing-Cong Li, Oxford, MS (US)
Assigned to TENFOLD TECHNOLOGIES, LLC, Pilot Point, TX (US); and UNIVERSITY OF MISSISSIPPI, University, MS (US)
Appl. No. 16/498,901
Filed by Tenfold Technologies, LLC, Pilot Point, TX (US); and University of Mississippi, University, MS (US)
PCT Filed Mar. 27, 2018, PCT No. PCT/US2018/024642
§ 371(c)(1), (2) Date Sep. 27, 2019,
PCT Pub. No. WO2018/183383, PCT Pub. Date Oct. 4, 2018.
Claims priority of provisional application 62/597,796, filed on Dec. 12, 2017.
Claims priority of provisional application 62/570,919, filed on Oct. 11, 2017.
Claims priority of provisional application 62/477,297, filed on Mar. 27, 2017.
Prior Publication US 2021/0085752 A1, Mar. 25, 2021
Int. Cl. A61K 38/16 (2006.01); C12N 1/20 (2006.01)
CPC A61K 38/164 (2013.01) [C12N 1/20 (2013.01)] 11 Claims
 
1. A method of treating a pathogen in a human, comprising administering to the human a composition comprising an effective amount of a cyclic fusaricidin compound and an open-chain fusaricidin compound,
wherein the cyclic fusaricidin compound has a mass range (m/z) value of less than 880.0 or wherein the cyclic fusaricidin compound has Formula I:

OG Complex Work Unit Chemistry
wherein X1 is Ser, X2 is Val or Ile, X3 is selected from the group consisting of Ile, Tyr, Val, and Phe, X4 is Thr or Ser, X5 is Asn or Gln, X6 is Ala,
wherein R1 is a 15-guanidino-3-hydroxypentadecanoyl (GHPD) side chain or 17-guanidino-3-(R)-hydroxyheptadecanoyl (GHHD) side chain, and
wherein the open-chain fusaricidin compound has Formula II:

OG Complex Work Unit Chemistry
wherein X7 is Thr or Ser; X8 is Val or Ile; X9 is selected from the group consisting of Val, Ile, Tyr, and Phe; X10 is Thr or Ser; X11 is Asn or Gln; X12 is selected from the group consisting of Ala, GABA, and Gly;
wherein R1 is a GHPD side chain,
and wherein the pathogen is (i) a bacterium selected from a Gram-positive bacterium, E. coli, and P. aeruginosa, (ii) a fungus, or (iii) a parasite selected from L. donovani and T brucei.