| CPC A61B 5/0071 (2013.01) [A61B 5/004 (2013.01); A61B 5/0095 (2013.01); A61B 5/407 (2013.01); A61B 5/4381 (2013.01); A61B 5/4893 (2013.01); A61B 90/37 (2016.02)] | 20 Claims |

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1. A method, comprising:
activating, by a surgical system that includes one or more devices, a voltage sensitive dye using neuromodulation,
wherein the voltage sensitive dye is activated based on the voltage sensitive dye binding to a cell membrane associated with a tissue of a patient, and
wherein activating the voltage sensitive dye causes a fluorescence and a photoacoustic effect;
obtaining, by the surgical system, imaging data regarding the tissue based on the fluorescence and the photoacoustic effect,
wherein the imaging data includes fluorescence images that depict the fluorescence and photoacoustic images that are based on the photoacoustic effect caused by activating the voltage sensitive dye, and
wherein the imaging data identifies a nerve map of at least one of:
one or more nerve bundles associated with the tissue of the patient, or
one or more nerve branches associated with the tissue of the patient;
causing, by the surgical system, the nerve map to be displayed,
wherein the displayed nerve map depicts different nerves using different colors based on one or more of a confidence level associated with a respective identified location of a nerve or a level of importance of a function of the nerve;
obtaining, by the surgical system and based on the voltage sensitive dye being activated using the neuromodulation, additional imaging data regarding the tissue over a threshold period of time after a performance of a surgical operation; and
determining, based on the additional imaging data, whether the tissue of the patient was damaged during the surgical operation.
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