US 12,274,279 B2
Methods for isolating compounds
Allan Otto Fog Lihme, Farum (DK); Marie Bendix Hansen, Frederiksberg (DK); and Bodil Ingrid Kjaer Lindved, Espergaerde (DK)
Assigned to DUYNIE HOLDING B.V., Alphen aan den Rijn (NL)
Filed by DUYNIE HOLDING B.V., Alphen aan den Rijn (NL)
Filed on Dec. 13, 2022, as Appl. No. 18/079,984.
Application 18/079,984 is a continuation of application No. 16/347,466, granted, now 11,553,724, previously published as PCT/DK2017/050365, filed on Nov. 7, 2017.
Claims priority of application No. PA 2016 70871 (DK), filed on Nov. 7, 2016; application No. PA 2017 70532 (DK), filed on Jun. 30, 2017; application No. PA 2017 70611 (DK), filed on Aug. 11, 2017; and application No. PA 2017 70677 (DK), filed on Sep. 11, 2017.
Prior Publication US 2023/0111991 A1, Apr. 13, 2023
Int. Cl. C07K 1/30 (2006.01); A23J 1/00 (2006.01); A23K 10/35 (2016.01); A23L 2/02 (2006.01); A23L 2/70 (2006.01); A23L 5/20 (2016.01); A23L 19/12 (2016.01); B01D 61/14 (2006.01); C07K 1/32 (2006.01); C07K 14/415 (2006.01)
CPC A23J 1/006 (2013.01) [A23K 10/35 (2016.05); A23L 2/02 (2013.01); A23L 2/70 (2013.01); A23L 5/20 (2016.08); A23L 5/276 (2016.08); A23L 19/12 (2016.08); B01D 61/145 (2013.01); C07K 1/30 (2013.01); C07K 1/32 (2013.01); C07K 14/415 (2013.01); A23V 2002/00 (2013.01); B01D 2311/12 (2013.01); B01D 2311/2642 (2013.01)] 20 Claims
 
1. A method for isolating a first group of compounds selected from one or more of patatin protein (PA), protease inhibitor protein (PI), lipoxygenase (LipO) and polyphenol oxidase (PPO) from a second group of compounds selected from one or more of PA, PI, LipO, PPO, glycoalkaloid and phenolic compounds, said method comprising:
a) providing an aqueous phase comprising compounds selected from two or more of PA, PI, PPO, LipO, glycoalkaloid and phenolic compounds of which at least one compound is selected from one or more of PA, PI, LipO and PPO;
b) contacting the aqueous phase with a mobile solubilized ligand at physico-chemical conditions allowing formation of a complex between the ligand and the compounds selected from one or more of PA, PI, LipO and PPO;
c) allowing the complex to separate from the aqueous supernatant phase, optionally by changing said physico-chemical conditions in the composition to reduce the stability of the complex; and
d) isolating the complex separated from the aqueous supernatant phase by precipitation.