US 11,953,502 B2
Thioredoxin 1 epitope, encoding nucleic acid and methods of binding a monoclonal antibody
Kyong Hoon Suh, Daejeon (KR); Dae Joong Kim, Seoul (KR); Young Kim, Cheongju-si (KR); Mi Kyung Kim, Daejeon (KR); Jong Hwan Jung, Daejeon (KR); and Ki Se Lee, Sejong (KR)
Assigned to E&S HEALTHCARE CO., LTD., Daejeon (KR)
Appl. No. 16/755,035
Filed by E&S HEALTHCARE CO., LTD., Daejeon (KR)
PCT Filed Oct. 12, 2018, PCT No. PCT/KR2018/012069
§ 371(c)(1), (2) Date Apr. 9, 2020,
PCT Pub. No. WO2019/074333, PCT Pub. Date Apr. 18, 2019.
Claims priority of application No. 10-2017-0132536 (KR), filed on Oct. 12, 2017.
Prior Publication US 2020/0309778 A1, Oct. 1, 2020
Int. Cl. C07K 7/08 (2006.01); C07K 7/00 (2006.01); C07K 7/06 (2006.01); C07K 14/00 (2006.01); C07K 16/40 (2006.01); G01N 33/543 (2006.01); G01N 33/574 (2006.01); G01N 33/577 (2006.01); G01N 33/58 (2006.01)
CPC G01N 33/57415 (2013.01) [C07K 7/08 (2013.01); C07K 16/40 (2013.01); G01N 33/54393 (2013.01); G01N 33/577 (2013.01); G01N 33/581 (2013.01); C07K 7/00 (2013.01); C07K 7/06 (2013.01); C07K 14/00 (2013.01); C07K 2317/21 (2013.01); C07K 2317/24 (2013.01); C07K 2317/34 (2013.01); C07K 2317/54 (2013.01); C07K 2317/55 (2013.01); C07K 2317/565 (2013.01); C07K 2317/622 (2013.01)] 11 Claims
 
1. An epitope recognized by a monoclonal antibody specifically binding to thioredoxin-1 (Trx1) or an antigen-binding fragment thereof, wherein the monoclonal antibody comprises:
a light chain variable region, which includes a light chain CDR1 consisting of the amino acid sequence of SEQ ID NO: 1, a light chain CDR2 consisting of the amino acid sequence of SEQ ID NO: 2 and a light chain CDR3 consisting of the amino acid sequence of SEQ ID NO: 3; and a heavy chain variable region, which includes a heavy chain CDR1 consisting of the amino acid sequence of SEQ ID NO: 4, a heavy chain CDR2 consisting of the amino acid sequence of SEQ ID NO: 5 and a heavy chain CDR3 consisting of the amino acid sequence of SEQ ID NO: 6; or
a light chain variable region which includes a light chain CDR1 consisting of the amino acid sequence of SEQ ID NO: 7, a light chain CDR2 consisting of the amino acid sequence of SEQ ID NO: 8 and a light chain CDR3 consisting of the amino acid sequence of SEQ ID NO: 9; and a heavy chain variable region, which includes a heavy chain CDR1 consisting of the amino acid sequence of SEQ ID NO: 10, a heavy chain CDR2 consisting of the amino acid sequence of SEQ ID NO: 11 and a heavy chain CDR3 consisting of the amino acid sequence of SEQ ID NO: 12,
wherein the epitope consists of any one amino acid sequence selected from SEQ ID Nos: 32 to 34 or 172, 174 and 175.