US 11,953,501 B2
Compositions and methods to detect gastrointestinal disease
Sunyoung Kim, New Orelans, LA (US)
Assigned to BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY AND AGRICULTURAL AND MECHANICAL COLLEGE, Baton Rouge, LA (US)
Appl. No. 17/428,416
Filed by The Board of Supervisors of Louisiana State University and Agricultural and Mechanical College, Baton Rouge, LA (US)
PCT Filed Feb. 4, 2020, PCT No. PCT/US2020/016646
§ 371(c)(1), (2) Date Aug. 4, 2021,
PCT Pub. No. WO2020/163381, PCT Pub. Date Aug. 13, 2020.
Application 17/428,416 is a continuation in part of application No. 16/267,120, filed on Feb. 4, 2019, granted, now 11,493,515.
Application 16/267,120 is a continuation in part of application No. PCT/US2017/045588, filed on Aug. 4, 2017.
Claims priority of provisional application 62/524,306, filed on Jun. 23, 2017.
Claims priority of provisional application 62/467,487, filed on Mar. 6, 2017.
Claims priority of provisional application 62/378,820, filed on Aug. 24, 2016.
Claims priority of provisional application 62/371,131, filed on Aug. 4, 2016.
Prior Publication US 2023/0036392 A1, Feb. 2, 2023
Int. Cl. G01N 33/53 (2006.01); G01N 33/573 (2006.01)
CPC G01N 33/573 (2013.01) [G01N 2333/916 (2013.01); G01N 2800/065 (2013.01); G01N 2800/52 (2013.01)] 4 Claims
 
1. A method for determining the prognosis of necrotizing enterocolitis (NEC) in an infant, the method comprising:
a) fitting a Markov model using a two state transition matrix and propensity values determined for a plurality of subjects, wherein the two state transition matrix comprises a first state and a second state, wherein the first state comprises a non-necrotizing enterocolitis status, wherein the second state comprises a necrotizing enterocolitis status, wherein a propensity value is a function of intestinal Alkaline Phosphatase (iAP) activity values and the amount of iAP found in a stool sample;
b) using a propensity value of the infant and the fitted Markov model to estimate a probability of transitioning from the first state to the second state, wherein the fitted model indicates that an increased propensity level of the infant significantly increases the probability of transitioning from the first state to the second state;
c) Identifying a propensity value of the infant that is greater than or equal to a threshold value of about 0.5, and treating the infant wherein the treating comprises withholding oral feeding, administering an antibiotic, a probiotic, an intravenous fluid, an iAP replacement composition, a small molecule effector of catalytic activity, an anti-inflammatory agent, parenteral or intravenous nutrition, a biologic, or a combination thereof.