US 11,953,498 B2
Image processing device, image processing method, program, and evaluation method
Tomoro Dan, Yokohama (JP); Hiroaki Kii, Fujisawa (JP); Takayuki Uozumi, Machida (JP); and Yasujiro Kiyota, Tokyo (JP)
Assigned to NIKON CORPORATION, Tokyo (JP)
Filed by NIKON CORPORATION, Tokyo (JP)
Filed on Apr. 12, 2021, as Appl. No. 17/227,967.
Application 17/227,967 is a continuation of application No. PCT/JP2019/039250, filed on Oct. 4, 2019.
Claims priority of application No. 2018-193814 (JP), filed on Oct. 12, 2018.
Prior Publication US 2021/0301246 A1, Sep. 30, 2021
Int. Cl. G06T 7/11 (2017.01); C12M 1/34 (2006.01); C12Q 1/04 (2006.01); G01N 33/50 (2006.01); G06T 7/00 (2017.01)
CPC G01N 33/5073 (2013.01) [C12M 41/36 (2013.01); C12Q 1/04 (2013.01); G06T 7/0016 (2013.01); G06T 7/11 (2017.01); G06T 2207/10056 (2013.01); G06T 2207/30024 (2013.01); G06T 2207/30242 (2013.01)] 14 Claims
OG exemplary drawing
 
1. An image processing device comprising:
a processor; and
a memory encoded with instructions executed by the processor, the instructions causing the processor to perform operations including:
imaging pluripotent stem cells over time in a culture process of the pluripotent stem cells and acquiring a plurality of images,
extracting a colony region of the pluripotent stem cells from an image of the plurality of images,
extracting, from the image, a high luminance region that is a region based on a group of pixels with larger luminance values than a standard value from among pixels constituting the image,
extracting an extraction target region with relatively high contrast in the colony region from the image based on the colony region and the high luminance region, and
outputting the extraction target region as an extraction result,
wherein as a relationship among the colony region, the high luminance region, and the extraction target region, the colony region is formed, the high luminance region is then formed in the colony region, and the extraction target region is then formed in the high luminance region in the culture process of the pluripotent stem cells, and
the extraction target region is a region where a striated pattern appears in the image.