US 11,953,479 B2
Selective optical aqueous and non-aqueous detection of free sulfites
Lynn Schmitt, Binghamton, NY (US); and Alistair Lees, Binghamton, NY (US)
Assigned to The Research Foundation for The State University of New York, Binghamton, NY (US)
Appl. No. 16/753,906
Filed by The Research Foundation for the State University of New York, Binghamton, NY (US)
PCT Filed Oct. 5, 2018, PCT No. PCT/US2018/054770
§ 371(c)(1), (2) Date Apr. 6, 2020,
PCT Pub. No. WO2019/071240, PCT Pub. Date Apr. 11, 2019.
Claims priority of provisional application 62/569,193, filed on Oct. 6, 2017.
Prior Publication US 2020/0363382 A1, Nov. 19, 2020
Int. Cl. G01N 31/22 (2006.01); C07D 401/04 (2006.01); G01N 21/78 (2006.01); G01N 33/14 (2006.01)
CPC G01N 31/22 (2013.01) [C07D 401/04 (2013.01); G01N 21/78 (2013.01); G01N 33/14 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method of detecting a free sulfite in an aqueous analyte, comprising:
reacting a first amount of the free sulfite in the aqueous analyte, with a compound having a 4-(1H-pyrrol-2-yl)pyridine ligand structure to perform a first bleaching;
measuring a first optical absorption corresponding to the first bleaching of the compound having the 4-(1H-pyrrol-2-yl)pyridine;
adding a second amount of the free sulfite in the aqueous analyte with the compound having the 4-(1H-pyrrol-2-yl)pyridine after the first bleaching, to perform a second bleaching;
measuring a second optical absorption corresponding to the second bleaching of the 4-(1H-pyrrol-2-yl)pyridine; and
calculating the free sulfite concentration in the aqueous analyte based on the measured first optical absorption and the measured second optical absorption.