US 11,953,464 B2
Semiconductor-based biosensors for base calling
Dietrich Dehlinger, San Francisco, CA (US); Ali Agah, Menlo Park, CA (US); Tracy Helen Fung, San Francisco, CA (US); and Emrah Kostem, Menlo Park, CA (US)
Assigned to Illumina, Inc., San Diego, CA (US)
Filed by ILLUMINA, INC., San Diego, CA (US)
Filed on Jun. 2, 2022, as Appl. No. 17/831,424.
Application 17/831,424 is a continuation of application No. 16/241,902, filed on Jan. 7, 2019, granted, now 11,378,544.
Claims priority of provisional application 62/614,930, filed on Jan. 8, 2018.
Claims priority of provisional application 62/614,934, filed on Jan. 8, 2018.
Claims priority of application No. 2020758 (NL), filed on Apr. 12, 2018.
Prior Publication US 2022/0299470 A1, Sep. 22, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 27/414 (2006.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01); C12Q 1/6869 (2018.01); C12Q 1/6874 (2018.01); G01N 21/64 (2006.01); G01N 21/76 (2006.01); G06F 18/24 (2023.01); G06V 10/75 (2022.01); G16B 30/00 (2019.01)
CPC G01N 27/4145 (2013.01) [B01L 3/502715 (2013.01); C12Q 1/6874 (2013.01); G01N 21/6454 (2013.01); G06F 18/24 (2023.01); G06V 10/751 (2022.01); B01J 2219/00711 (2013.01); B01J 2219/00722 (2013.01); B01L 7/52 (2013.01); B01L 2200/147 (2013.01); B01L 2300/0636 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/0663 (2013.01); B01L 2300/0893 (2013.01); C12Q 1/6869 (2013.01); G01N 2021/6432 (2013.01); G01N 2021/6441 (2013.01); G01N 2021/6471 (2013.01); G01N 2021/6478 (2013.01); G01N 2021/6482 (2013.01); G01N 21/76 (2013.01); G01N 21/763 (2013.01); G01N 2223/40 (2013.01); G01N 2223/413 (2013.01); G05B 2219/37563 (2013.01); G16B 30/00 (2019.02)] 11 Claims
OG exemplary drawing
 
1. A device for base calling, comprising:
a receptacle configured to hold a biosensor, the biosensor having
a sample surface that holds a plurality of clusters during a sequence of sampling events,
an array of sensors, where each sensor in the array senses information from one or more clusters disposed in corresponding pixel areas of the sample surface to generate a pixel signal in a sampling event, the array configured to generate a plurality of sequences of pixel signals, the array having a number N of active sensors, the sensors in the array disposed relative to the sample surface to generate respective pixel signals during the sequence of sampling events from the number N of corresponding pixel areas of the sample surface to produce the plurality of sequences of pixel signals, and
a communication port which outputs the plurality of sequences of pixel signals; and
a signal processor coupled to the receptacle, and configured to receive and to process the plurality of sequences of pixel signals to classify results of the sequence of sampling events on clusters in the plurality of clusters, wherein information sensed from at least two clusters in the corresponding pixel area is additively combined and represented in a common pixel signal for each sampling event in at least one sequence of pixel signals in the plurality of sequences of pixel signals, and including using the plurality of sequences of pixel signals to classify results of the sequence of sampling events on a number N+M of clusters in the plurality of clusters from the number N of active sensors, where M is a positive integer.