US 11,952,687 B2
Method for producing cell-culturing polyvinyl alcohol nanofiber structure
Jong Young Kwak, Suwon-si (KR); Jung Min Kim, Suwon-si (KR); Yeo Jin Park, Bucheon-si (KR); Dan Bi Park, Seongnam-si (KR); Qasaim Muhammad, Suwon-si (KR); and Young Hun Jeong, Daegu (KR)
Assigned to NANOFAENTECH CO., LTD., Gimhae-si (KR)
Appl. No. 16/319,148
Filed by NANOFAENTECH CO., LTD., Gimhae-si (KR)
PCT Filed Jan. 12, 2017, PCT No. PCT/KR2017/000393
§ 371(c)(1), (2) Date Jan. 18, 2019,
PCT Pub. No. WO2018/016701, PCT Pub. Date Jan. 25, 2018.
Claims priority of application No. 10-2016-0091502 (KR), filed on Jul. 19, 2016.
Prior Publication US 2021/0332511 A1, Oct. 28, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. D04H 1/4309 (2012.01); C12N 5/00 (2006.01); D01D 5/00 (2006.01); D01D 10/02 (2006.01); D01D 10/06 (2006.01); D04H 1/728 (2012.01); D06M 11/11 (2006.01); D06M 13/127 (2006.01); D06M 13/402 (2006.01)
CPC D04H 1/4309 (2013.01) [C12N 5/0068 (2013.01); D01D 5/003 (2013.01); D01D 5/0038 (2013.01); D01D 5/0069 (2013.01); D01D 10/02 (2013.01); D01D 10/06 (2013.01); D04H 1/728 (2013.01); D06M 11/11 (2013.01); D06M 13/127 (2013.01); D06M 13/402 (2013.01); C12N 2533/30 (2013.01)] 6 Claims
OG exemplary drawing
 
1. A method for producing a cell-culturing polyvinyl alcohol-based nanofiber structure, the method comprising:
electrospinning an electrospun solution to form a nanofiber mat, wherein the electrospun solution contains polyvinyl alcohol (PVA), polyacrylic acid (PAA) and glutaraldehyde (GA);
crosslinking the nanofiber mat via a hydrochloric acid (HCl) vapor treatment; and
treating the crosslinked nanofiber mat with dimethylformamide (DMF) solvent to crystallize the nanofiber mat.