	US 11,952,630 B2
	Dried compositions containing flap endonuclease
Patrick Peterson, San Marcos, CA (US); Tony Luu, San Diego, CA (US); and Matthias Jost, San Diego, CA (US)
Assigned to GEN-PROBE INCORPORATED, San Diego, CA (US)
Filed by Gen-Probe Incorporated, San Diego, CA (US)
Filed on May 17, 2022, as Appl. No. 17/746,400.
Application 17/746,400 is a continuation of application No. 17/674,521, filed on Feb. 17, 2022, abandoned.
Application 17/674,521 is a continuation of application No. 16/613,744, granted, now 11,286,526, issued on Mar. 29, 2022, previously published as PCT/US2018/033549, filed on May 18, 2018.
Claims priority of provisional application 62/540,478, filed on Aug. 2, 2017.
Claims priority of provisional application 62/508,990, filed on May 19, 2017.
Claims priority of provisional application 62/508,975, filed on May 19, 2017.
Prior Publication US 2022/0275448 A1, Sep. 1, 2022
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6844 (2018.01); C12Q 1/6876 (2018.01)

CPC C12Q 1/6876 (2013.01) [C12Q 1/6844 (2013.01); C12Q 2527/125 (2013.01); C12Y 207/07007 (2013.01)]

21 Claims

1. A kit for use in performing a nucleic acid based assay, the kit comprising a first vessel containing adried composition, and a second vessel containing a reconstitution solution; wherein the dried composition comprises a bulking agent, a flap-endonuclease, dNTPs, a chelating agent, 3-(N-morpholino)propanesulfonic acid (MOPS) or tris(hydroxymethyl)aminomethane (Tris), and contains substantially no magnesium ions; and wherein the reconstitution solution comprises MgCl₂at a concentration from about 3.8 mM to about 4.4 mM.

21. A kit for use in performing a non-nucleic acid based assay, the kit comprising a first vessel containing a dried composition and a second vessel containing a reconstitution solution; wherein the dried composition comprises a bulking agent, a flap-endonuclease, a DNA polymerase, dNTPs, a chelating agent, 3-(N-morpholino)propanesulfonic acid (MOPS) or tris(hydroxymethyl)aminomethane (Tris), and contains substantially no magnesium ions; and wherein the reconstitution solution comprises MgCl₂at a concentration from about 3.8 mM to about 4.4 mM.