US 11,952,616 B2
Methods and systems for analyzing nucleic acid molecules
Andrew Kennedy, San Diego, CA (US); Stefanie Ann Ward Mortimer, Morgan Hill, CA (US); Helmy Eltoukhy, Atherton, CA (US); AmirAli Talasaz, Atherton, CA (US); and Diana Abdueva, Orinda, CA (US)
Assigned to Guardant Health, Inc., Palo Alto, CA (US)
Filed by GUARDANT HEALTH, INC., Palo Alto, CA (US)
Filed on Dec. 5, 2022, as Appl. No. 18/061,898.
Application 18/061,898 is a continuation of application No. 16/450,918, filed on Jun. 24, 2019, granted, now 11,519,019.
Application 16/450,918 is a continuation of application No. PCT/US2017/068329, filed on Dec. 22, 2017.
Claims priority of provisional application 62/438,240, filed on Dec. 22, 2016.
Claims priority of provisional application 62/512,936, filed on May 31, 2017.
Claims priority of provisional application 62/550,540, filed on Aug. 25, 2017.
Prior Publication US 2023/0332206 A1, Oct. 19, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/68 (2018.01); C12N 15/10 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6874 (2018.01); C12Q 1/6886 (2018.01)
CPC C12Q 1/6806 (2013.01) [C12N 15/1065 (2013.01); C12Q 1/6874 (2013.01); C12Q 1/6886 (2013.01); C12Q 2600/154 (2013.01)] 21 Claims
 
1. A method of analyzing nucleic acids obtained from a subject having cancer or suspected of having cancer, comprising:
(a) purifying cell-free nucleic acids from a bodily fluid sample obtained from the subject;
(b) physically fractionating the cell-free nucleic acids to generate two or more partitions, wherein the physical fractionating comprises fractionating nucleic acids based on one or more characteristics, wherein the one or more characteristics comprises methylation status; and
(c) sequencing at least a portion of nucleic acids from each of the two or more partitions to generate a set of sequencing reads.